Figure 6.

Reversibility of sperm-egg fusion inhibition by PSG17NMyc-His. Zona-free eggs were incubated for 30 min in medium containing PSG17N-Myc-His (50 μg/ml), washed through 3 drops of medium, and then inseminated with capacitated sperm (PSG17 + wash). Control groups consisted of oocytes incubated in medium (Control), or in the presence of PSG17N-Myc-His without its subsequent removal by washing (PSG17). After coincubating the gametes for 40 min, oocytes were washed and the fertilization rate and the fertilization index were scored. Data represents the mean value ± SE from three independent experiments. ^*p < 0.05 compared with control and to PSG17 + wash.