Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 23;123(8):1352–1362. doi: 10.1242/jcs.056176

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010.

PMC Copyright notice

Fig. 2. — KSRP-Dvl3 interaction is mediated through the C-terminus of Dvl3. (A) F9 cells were treated with 100 nM Dvl3 siRNA for 24 hours followed by transient expression of either HA-hDvl3 or HA-hDvl3-ΔCTM (1-496) or HA-Dvl3-CTM (497-716) alone or together with FLAG-KSRP for 24 ho urs followed by cell lysis and affinity pull-downs with anti-HA-affinity matrix. Interaction of KSRP with Dvl3 or its mutants was visualized by probing the blots with anti-FLAG antibody. Asterisks indicate the bands of immunoglobulin hea vy and light chains. (B) F9 cells were transiently transfected with either HA-Dvl1-GFP2 or HA-Dvl2-GFP2 or HA-Dvl3-GFP2 alone or with FLAG-KSRP for 24 hours followed by cell lysis and affinity pull-downs with anti-HA-affinity matrix. Interaction of KSRP with Dvl isoforms was visualized by probing the blots with anti-FLAG antibody. (C) HEK293 cells were transiently transfected with HA-Dvl3-GFP2 alone or with FLAG-KSRP for 24 hours followed by cell lysis and affinity pull-downs with anti-HA-affinity matrix. Interaction of KSRP with Dvl3 was visualized by probing the blots with anti-FLAG antibody. Representative blots of three independent experiments that proved highly reproducible are displayed.