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. Author manuscript; available in PMC: 2011 Mar 26.
Published in final edited form as: Mol Cell. 2010 Mar 26;37(6):879–886. doi: 10.1016/j.molcel.2010.01.036

Figure 3. DNA binding by the MHF1-MHF2 heterodimer.

Figure 3

(A) Recombinant MHF1-(His)6-MHF2, MBP-MHF1, and MBP-MHF2 were expressed in and purified from E. coli. Purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining. (B) MHF1-(His)6-MHF2, MBP-MHF1 (1.0 and 2.0 μM), and MBP-MHF2 (1.0 and 2.0 μM) were examined for their DNA binding attribute with a mixture of the four 32P-labeled DNA substrates. (C). The indicated combinations of DNA substrates were incubated with MHF1-(His)6-MHF2 (50, 85, 120, 155, 190, 225, 270 and 305 nM). Quantification of the data is presented in the right panels. Note that treatment of nucleoprotein complexes with SDS and proteinase K (SDS/PK) released the DNA substrates. See also Fig. S3.