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. Author manuscript; available in PMC: 2011 Mar 26.
Published in final edited form as: Mol Cell. 2010 Mar 26;37(6):879–886. doi: 10.1016/j.molcel.2010.01.036

Figure 4. Enhancement of DNA branch migration by MHF1-MHF2 heterodimer.

Figure 4

(A) Amylose beads pull-down assay with FANCM and MBP-MHF1 or MBP-MHF2. S, W, and E represent supernatant, wash and elution lanes. The asterisk (*) denotes a proteolytic product of FANCM. (B) Stimulation of the branch migration activity of FANCM by the MHF1-MHF2 dimer. ATP was present in all the reactions except the one in lane 8. NP - no protein control; BM, branch migration. (C) Quantification of the results shown in figure 4B. (D) DNA branch migration by FANCM, FANCMK117R, or the combination of FANCM or FANCMK117R and MHF1-MHF2 was examined with or without ATP, ATP-γ-S, or AMP-PNP, as indicated. BM, branch migration. (E) ATP hydrolysis by FANCM, MHF1-MHF2 or FANCMK117R was examined with or without ssDNA, as indicated. (F) Effect of MHF1-MHF2 on the ssDNA-dependent ATPase activity of FANCM. See also Fig. S3 and S4.