(A) Amylose beads pull-down assay with FANCM and MBP-MHF1 or MBP-MHF2. S, W, and E represent supernatant, wash and elution lanes. The asterisk (*) denotes a proteolytic product of FANCM. (B) Stimulation of the branch migration activity of FANCM by the MHF1-MHF2 dimer. ATP was present in all the reactions except the one in lane 8. NP - no protein control; BM, branch migration. (C) Quantification of the results shown in figure 4B. (D) DNA branch migration by FANCM, FANCMK117R, or the combination of FANCM or FANCMK117R and MHF1-MHF2 was examined with or without ATP, ATP-γ-S, or AMP-PNP, as indicated. BM, branch migration. (E) ATP hydrolysis by FANCM, MHF1-MHF2 or FANCMK117R was examined with or without ssDNA, as indicated. (F) Effect of MHF1-MHF2 on the ssDNA-dependent ATPase activity of FANCM. See also Fig. S3 and S4.