Table 2.
Detail information for primers of the chicken DRD1 gene
| Primer name | Primer sequence (5'→3') |
Length1 (bp) | Location2 | AT3 (°C) | Genotyping method | |
|---|---|---|---|---|---|---|
| P1 | F:CCGGTGAGTACCCTGCTTT R:GTGCTTTTCCTCTGCTTTGG |
1504 | -1961 ~ -458 | 59 | / | |
| P2 | F:AGTGAAGAATTGCTCGCTGA R:GGTTTTGCTGGGTACACCTT |
1970 | -589 ~ +1381 | 57 | / | |
| P3 | F:CACTATGGATGGGGAAGGGTTG R: GGCCACCCAGATGTTGCAAAATG |
283 | G+123A T+198C |
62 |
BseNI cfrI |
|
| P4 | F: CAGCCCATTCAGGTACGAGAGGA R: ATTCGACTCTTTGGGGCTGGAC |
793 | A+505G C+765T C+1011T G+1065A C+1107T |
65.5 | sequencing | |
| P5 | F: TTTCCTTCATCCCCGTGCAGCT R: GCTGCTTCTGTTGCCACTTGTGT |
290 | +458 ~ +747 | 63 | / | |
| P6 (actin) | F: CCCCAAAGCCAACAGAGAGA R: GGTGGTGAAGCTGTAGCCTCTC |
274 | / | 63 | / | |
1The length of PCR products; 2referred to the locations in the DRD1 gene, the first nucleotide of translation start codon was designated as +1. 3indicated annealing temperature.