Table 1.
Summary of reported yields obtained with the λpL/pR/cI857 thermoinduced expression system.
| Protein | Plasmid | Culture conditions; induction strategy | Production | System | Ref. |
|---|---|---|---|---|---|
| Recombinant protein localized in cytoplasm | |||||
| UVRA | pGHY5003 | BC; 30 to 42°C | 7% TCP | λpl, cI857 | [44] |
| IFN-β | pPLc245-HFIF25 | BC; 28 to 42°C | 4% TCP | λpL/cI857 | [61] |
| GXP | pHEGPT | BC, SF; 30 to 42°C | 5% TCP | λpL/cI857 | [58] |
| IHF α, β | pPlhima-1, pPLhipohim A-s | BC; 31 to 42°C | 3 mg/L | λpL/cI857 | [169] |
| rpGH | p-LARpGH | BC, SF; 30 to 42°C (3-4 h), temp reached in 5-8 min | 15% TCP | λpL/lcI857 | [170] |
| rpGH | p-LARpGH | FB; 30 to 42°C | 20% TCP | λpL/lcI857 | [170] |
| β-gal | pJLACZ | CC; 28 to 40°C | 3,037 U/ml/OD | λpL cI857 | [16] |
| β-gal | pJLACZ | CC; 28 to 42°C | 7,327 U/ml/OD | λpL cI857 | [16] |
| SpA-β-gal | BC; 30 to 40°C | 6.2 g/L | λpR | [65] | |
| SpA-β-gal | FB; 30 to 40°C | 19.2 g/L | λpR | [65] | |
| β-gal | pACYC177; pRSET- lacZ |
BC, SF; 30 to 42°C (2 min), reducing to 37°C | 23500 U | λpL/cI857 | [171] |
| β-gal | pACYC177; pRSET- lacZ |
BC, SF- FB; 30 to 42°C (2 min), reducing to 37°C | 23000 U | λpL/cI857 | [171] |
| β-gal | pACYC177; pRSET- lacZ |
FB; 30 to 42°C (2 min), reducing to 37°C | 285000 U (0.95 g/L) |
λpL/cI857 | [171] |
| Carbamoylase | pTAH10; pT-GroE |
BC, SF; 30 to 39°C reached abruptly (10 to 20 min), decrease to 37°C | 7 U (0.14 U/mL) |
λpLpR, cI857-T7 RNA pol | [67] |
| Carbamoylase | pTAH10; pT-GroE |
BC; 30-39°C (10 to 20 min), decrease to 37°C | 1830 U (1.2 U/mL) |
λpLpR, cI857-T7 RNA pol | [67] |
| Carbamoylase | pTAH10; pT-GroE |
FB; 30 to 39°C (10 min), decrease to 37°C | 14256 U (5.8 U/mL) |
λpLpR, cI857-T7 RNA pol | [67] |
| TNF-α | pCY-TNF | BC, SF; 30 to 42°C | 12% soluble TCP | λpRpL cI 857 |
[10] |
| TNF-α | pCY-TNF | BC, 30 to 42°C (6 h) | 11% soluble TCP | λpRPL cI 857 |
[10] |
| TRAIL | pBV-Trail | FB; 30 to 42°C (4 h) | 1.4 g/L | Temperature inducible | [172] |
| GFP | pND-GFP | BC; 30 to 42°C (1 h), reducing to 30°C |
30 mg/L | λpL/cI857 | [137] |
| GFP | pND-GFP | BC; 37 to 42°C (1 h), reducing to 37°C |
50 mg/L | λpL/cI857 | [137] |
| GFP | pND-GFP | BC, SF; 37 to 42°C (1 h), reducing to 37°C |
7 mg/L | λpL/cI857 | [137] |
| GFP | pND-GFP | BC, SF; 37 to 42°C (1 h), reduction to 37°C (1 h), increasing to 42°C (1 h), reducing to 37°C | 68 mg/L | λpL/cI857 | [137] |
| GFP | pND-GFP | BC, SF; 30 to 42°C (1 h), reduction to 30°C (1 h), increase to 42°C (1 h), decrease to 30°C |
45 mg/L | λpL/cI857 | [137] |
| GFP | pND-GFP | FB; 30 to 42°C (30 min), decrease to 30°C, increase to 42°C (30 min), decrease to 30°C |
273 mg/L | λpL/cI857 | [137] |
| IB localized in cytoplasm | |||||
| IFN-γ | pPL-l | BC, SF; 28°C-42°C | 0.3 g/L | λpL/cI857 | [52] |
| IFN-γ | pPL-l | FB; 28 to 42°C | 7.43 g/L | λpL/cI857 | [52] |
| Insulin A- chain-Mut3sY | PMYW-A | BC, SF: BL21; 30 to 42°C | 30% TCP | λpl/cI857 | [53] |
| Insulin A- chain-Mut3sY | PMYW-A | FB, HCDC; 30 to 42°C | 13% TCP | λpl, lcI857 | [53] |
| Insulin B- chain- Mut3sY | PMYW-B | FB, HCDC; 30 to 42°C | 18% TCP (4.6 g/L) |
λpl, lcI857 | [53] |
| IFN-α | pMYW-a | FB; 30 to 42°C | 4 g/L | λpl, lcI857 | [7] |
| rhBMP-2 | pCYTEXP3-BMP-2 | BC, SF 30 to 42°C | 25% TCP | λpL, cI857 | [94] |
| rhBMP-2 | pCYTEXP3-BMP-2 | FB; 30 to 42°C | 20% TCP (8.5 g/L) |
λpL, cI857 | [94] |
| hFGF-2 | plFGFB | FB; 30 to 42°C (6-8 h) | 5-6 g/L | λpRPL cI857 | [95] |
| hGH | pET21-hgh | FB (complex media); 30 to 42°C (30 min), reduction to 37°C (4 h) |
11% TCP (2.0 g/L) |
pET21-hgh; pGP1-2 gene 1 of T7 under λpL | [54] |
| hGH | pET21-hgh | FB (glycerol); 30 to 42°C (30 min), reduction to 37°C (4 h) |
15% TCP (2.7 g/L) |
pET21-hgh; pGP1-2 gene 1 of T7 under λpL | [54] |
| IFN-α 2b | pRSET-INFα 2b; pGP1-2 | BC, SF; 30 to 42°C (5, 10 and 15 min) |
650 mg/L | λPL, T7 RNA pol, cI857 | [55] |
| His-tag-hPPI | λ-PL-cI857 pUC | FB; 30 to 42°C heat rate 0.4°C/min |
3.3 g/L | λpL/cI857 | [15] |
| His-tag- hPPI | λ-PL-cI857 pUC | FB; 30 to 42°C heat rate 0.8°C/min |
2.2 g/L | λpL/cI857 | [15] |
| His tag- hPPI | λ-PL-cI857 pUC | FB; 30 to 42°C heat rate 1.7°C/min |
1.8 g/L | λpL/cI857 | [15] |
| His tag- hPPI | λ-PL-cI857 pUC | FB; 30 to 42°C heat rate 6°C/min |
1.9 g/L | λpL/cI857 | [15] |
| Recombinant protein localized in cytoplasm and supernatant | |||||
| TK | pHETK2 | BC, SF; 30°C to 42°C (16 h) | 4% soluble TCP | λpL/cI857 | [50] |
| TK | pHETK2 | BC, SF; 30°C to 42°C (16 h) | 4% soluble TCP | λpL/cI857 | [50] |
| β-gal | pRA-A1Its-187Z | BC, SF; 30 to 40°C (5 h) | 30% TCP (2.0 g/L) |
λpR, cI857, PA1 | [66] |
| β-gal | pRA-A1Its-187Z | BC; 30 to 40°C | 22% TCP (2.0 g/L) |
λpR, cI857, PA1 | [66] |
| scFv | pCMT2b-scFv;pRcd1 | BC, SF; 30°C to 42°C (7 h) | 37 mg/L | λpL/cI857; Rcd lPR | [173] |
| scFv | pCMT2b-scFv;pRcd1 | FB; 30 to 42°C (8 h) | 160 mg/L | λpL/cI857; Rcd IPR | [173] |
| Recombinant protein localized in supernatant | |||||
| Glucagon-SEAP | pBLGlu-2 | BC, SF; 30 to 40°C | 3.4 mg/L/OD600 | λpL/cI857 | [174] |
| Recombinant protein localized in periplasm | |||||
| hGH-fusion to DsbA | DsbA-hGH | BC, SF; 30 to 42°C | 19% | λpL/cI857 | [125] |
| hGH | lPL-DsbA-hGH/pRK248cIts | FB; 30 to 42°C heat rate 6°C/min |
95 mg/L | λpL -cI857 | [142] |
UVRA: UV resistant-isoform A; IHF: Integration host factor; IFN: interferon; GXP: Guanine-xanthine phosphoribosyltransferase; rpGH: recombinant porcine growth hormone; β-gal: β-galactosidase; SpA: staphylococcal protein; TNF: tumor necrosis factor; TRAIL: TNF-related apoptosis-inducing ligand; GFP: green fluorescent protein; Mut3sY: Shorter version of INF-g gene; rhBMP-2: recombinant human bone morphogenetic protein 2; hFGF-2: human fibroblast growth factor 2; hGH: human growth hormone; scFv: single chain variable fragment; hPPI: Human pre-proinsulin; TK: Thymidine kinase; SEAP alkaline phosphatase; λPL: bacteriophage lambda; IB: Inclusion bodies; TCP: total cell protein; SF: shake flasks; HCDC: high cell density cultures; BC: Batch culture; FB: Fed-batch culture; CC: Continuous culture.