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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Free Radic Biol Med. 2009 Nov 24;48(4):493–498. doi: 10.1016/j.freeradbiomed.2009.11.012

Fig. 3. H2O2 is the major ROS produced by endothelial cell-associated XO.

Fig. 3

BAEC were exposed to XO (5 mU/ml) as described in the methods. Cell suspensions (2 × 106 cells /ml) were monitored for O2 consumption upon the addition of xanthine. Either SOD (200 U/ml), CAT (200 U/ml) or both were added to the samples before xanthine as indicated. The effects of the presence of (CAT, SOD, CAT+SOD) are represented as % decrease (inset) in the rate of O2 consumption from control samples without antioxidant and are plotted for the indicated O2 tensions. Values represent the mean of 3 independent determinations. (*) indicates p < 0.05 compared to CAT only and/or CAT+SOD.