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Mannosidase assays of wild-type and mutant Caenorhabditis. Pyridylaminated Man5Gn was incubated either with no enzyme (A) or with either wild-type (B) or tm1078 (C) extracts overnight at 30 °C prior to RP-HPLC. The tm1078 extract displays no α-mannosidase II activity compatible with deletion of part of the aman-2 gene in this strain.
