Table 1. Summary of MS data for underivatised glycans from the N2 and tm1078 strains.
Monoisotopic [M+Na+] m/z values from the spectra presented in Figure 5 (panels A and B) and the corresponding hypothesised compositions are shown for PNGase A-released N-glycans from wild-type N2 and mutant tm1078 aman-2 strains. ND, not detected. Abundances for detected glycans refer to percentage intensities relative to the peak of highest intensity (for N2 this is the m/z 933 peak and for tm1078 the m/z 1460 peak).
| Putative composition | N2 | tm1078 | ||
|---|---|---|---|---|
| m/z | abundance | m/z | abundance | |
| Fuc1Hex2HexNAc2 | 917.27 | 10 | ND | |
| Fuc1Hex2HexNAc2Me1 | 931.31 | 13 | ND | |
| Hex3HexNAc2 | 933.39 | 100 | 933.52 | 3 |
| Fuc2Hex2HexNAc2 | 1063.44 | 13 | ND | |
| Fuc2Hex2HexNAc2Me1 | 1077.27 | 14 | ND | |
| Fuc1Hex3HexNAc2 | 1079.46 | 81 | ND | |
| Fuc1Hex3HexNAc2Me1 | 1093.37 | 7 | ND | |
| Hex4HexNAc2 | 1095.46 | 33 | 1095.64 | 10 |
| Hex3HexNAc3 | 1136.54 | 22 | ND | |
| Fuc2Hex3HexNAc2 | 1225.59 | 16 | ND | |
| Fuc2Hex3HexNAc2Me1 | 1239.61 | 11 | 1239.73 | 8 |
| Fuc1Hex4HexNAc2 | 1241.56 | 17 | 1241.73 | 5 |
| Fuc1Hex4HexNAc2Me1 | 1255.62 | 10 | 1255.71 | 28 |
| Hex5HexNAc2 | 1257.59 | 43 | 1257.74 | 94 |
| Fuc1Hex3HexNAc3 | 1282.61 | 8 | ND | |
| Hex3HexNAc3PC1 | 1301.45 | 6 | ND | |
| Hex3HexNAc4 | 1339.62 | 6 | ND | |
| Fuc3Hex3HexNAc2 | 1371.66 | 7 | ND | |
| Fuc3Hex3HexNAc2Me1 | 1385.67 | 8 | ND | |
| Fuc2Hex4HexNAc2 | 1387.66 | 17 | ND | |
| Fuc2Hex4HexNAc2Me1 | 1401.63 | 14 | 1401.80 | 5 |
| Fuc1Hex5HexNAc2 | 1403.55 | 9 | 1403.84 | 9 |
| Fuc1Hex5HexNAc2Me1 | 1417.58 | 5 | 1417.84 | 15 |
| Hex6HexNAc2 | 1419.62 | 20 | 1419.84 | 28 |
| Fuc1Hex5HexNAc2Me2 | ND | 1431.86 | 5 | |
| Hex6HexNAc2Me1 | ND | 1433.85 | 4 | |
| Fuc1Hex3HexNAc3PC1 | 1447.54 | 3 | ND | |
| Fuc1Hex4HexNAc3Me1 | ND | 1458.86 | 27 | |
| Hex5HexNAc3 | ND | 1460.87 | 100 | |
| Fuc1Hex3HexNAc4 | 1485.32 | 6 | ND | |
| Hex3HexNAc4PC1 | 1504.56 | 3 | ND | |
| Fuc3Hex4HexNAc2 | 1533.69 | 13 | ND | |
| Fuc3Hex4HexNAc2Me1 | 1547.40 | 9 | ND | |
| Fuc2Hex5HexNAc2 | 1549.75 | 8 | ND | |
| Fuc2Hex5HexNAc2Me1 | 1563.66 | 5 | 1563.92 | 10 |
| Fuc1Hex6HexNAc2 | ND | 1565.91 | 7 | |
| Fuc2Hex5HexNAc2Me2 | ND | 1577.94 | 5 | |
| Fuc1Hex6HexNAc2Me1 | ND | 1579.92 | 13 | |
| Hex7HexNAc2 | 1581.68 | 19 | 1581.92 | 22 |
| Fuc2Hex4HexNAc3Me1 | ND | 1604.93 | 11 | |
| Fuc1Hex5HexNAc3 | ND | 1606.95 | 34 | |
| Fuc1Hex5HexNAc3Me1 | ND | 1620.93 | 11 | |
| Hex6HexNAc3 | ND | 1622.95 | 32 | |
| Hex5HexNAc3PC1 | ND | 1625.95 | 10 | |
| Fuc1Hex5HexNAc3Me2 | ND | 1634.96 | 4 | |
| Hex6HexNAc3Me1 | ND | 1636.97 | 9 | |
| Fuc4Hex4HexNAc2 | 1679.79 | 5 | ND | |
| Fuc4Hex4HexNAc2Me1 | 1693.65 | 7 | ND | |
| Fuc3Hex5HexNAc2 | 1695.61 | 14 | ND | |
| Fuc3Hex5HexNAc2Me1 | 1709.33 | 6 | ND | |
| Fuc2Hex6HexNAc2 | 1711.53 | 4 | ND | |
| Fuc2Hex6HexNAc2Me1 | ND | 1726.00 | 8 | |
| Fuc1Hex7HexNAc2 | ND | 1728.01 | 4 | |
| Fuc1Hex7HexNAc2Me1 | ND | 1741.99 | 10 | |
| Hex8HexNAc2 | 1743.68 | 25 | 1743.99 | 23 |
| Fuc1Hex6HexNAc3 | ND | 1769.03 | 15 | |
| Fuc1Hex5HexNAc3PC1 | ND | 1772.02 | 6 | |
| Fuc2Hex5HexNAc3Me2 | ND | 1781.02 | 5 | |
| Fuc1Hex6HexNAc3Me1 | ND | 1783.04 | 15 | |
| Fuc1Hex6HexNAc3Me2 | ND | 1797.09 | 5 | |
| Fuc4Hex5HexNAc2 | 1841.70 | 4 | ND | |
| Fuc4Hex5HexNAc2Me1 | 1855.61 | 6 | ND | |
| Fuc3Hex6HexNAc2 | 1857.82 | 5 | ND | |
| Fuc2Hex7HexNAc2Me1 | ND | 1888.09 | 3 | |
| Fuc1Hex8HexNAc2Me1 | 1903.42 | 3 | 1904.05 | 10 |
| Hex9HexNAc2 | 1905.63 | 32 | 1906.06 | 35 |
| Fuc2Hex6HexNAc3Me1 | ND | 1929.11 | 9 | |
| Fuc1Hex7HexNAc3 | ND | 1931.10 | 4 | |
| Fuc4Hex6HexNAc2Me1 | 2017.65 | 2 | ND | |
| Hex10HexNAc2 | 2067.54 | 2 | 2068.13 | 2 |
| Fuc3Hex6HexNAc3Me2 | ND | 2089.15 | 2 | |
| Fuc2Hex7HexNAc3Me1 | ND | 2091.18 | 3 | |