Table 2. List and sequence of primers used for studying daf-16 expression levels using Real Time PCR.

Gene	Species	Forward Primer 5′	Reverse Primer 3′
daf-16	C. elegans	GCGAATCGGTTCCAGCAATTCCAA	ATCCACGGACACTGTTCAACTCGT
daf-16	C. briggsae	AGAAGGCTACCACTAGAACCAACG	TCCATCCAGCGGAACTGTTCGAAT
daf-16	C. remanei	CGACGGCAATACTCATGTCAATGG	ACGGTTTGAAGTTGGTGCTTGGCA
daf-16	C. brenneri	CCTTAGTAGTGGCCTCAATGGTGT	CACAACCTATCACTTCACTCTCGC
gpd-3	All species	TGAAGGGAATTCTCGCTTACACC	GAGTATCCGAACTCGTTATCGTAC