Fig. 3.

a Results of trypan blue exclusion assay in a typical experiment demonstrating that co-treatment with vincristine (VCR) markedly decreases doxorubicin (DOX)-induced cardiomyocyte death. b Results of a live/dead assay. With doxorubicin treatment alone for 24 h there was an increased proportion of dead cells (ethidium positive, EthD/red fluorescence) and a decreased proportion of live cells (calcein ester positive/green fluorescence) compared to vehicle treatment. With co-treatment with vincristine there were more live than dead cells. (Published with permission from Chatterjee et al. [58])