Figure 3. 6a-P peptide inhibits endothelial cells migration.

HUVECs were plated into inserts (8 µm pore size) of 24-well transwell plate and duplicated. The lower chamber was filled with serum free EBM medium containing 50 ng/ml rhVEGF plus different concentrations of the peptide for 16 h. The results show that endothelial cells migrating across the membrane are suppressed by the peptide in dose-dependent manner (A). VEGF induces endothelial cell migration is inhibited almost 90% when peptide is present at high concentration (B; P<0.001).