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. 2010 Apr 1;5(4):e9951. doi: 10.1371/journal.pone.0009951

Figure 6. Antisense oligonucleotides decreased Prdm1 immunostaining and inhibited expression of fast and slow MyHCs in somite explant cultures.

Figure 6

Somite tissues were dissected from E4 embryos (HH stage 23–25), cultured for 2 days, and analyzed for expression of Prdm1 and MyHC isoforms. A, C, E. In the presence of a non-specific oligonucleotide, cells derived from the main explant formed a surrounding monolayer of cells (A, nuclei) and both Prdm1-positive cells (C, Ab from Cell Signaling Technology) and MyHC-expressing myocytes (E, S58-positive) were found among these cells. The main tissue explant (exp) is to the lower right of each panel. B, D, F. In the presence of Prdm1 antisense nucleotides, formation of the monolayer was not affected (B, nuclei), but expression of both Prdm1 (C, Ab from Cell Signaling Technology) and slow MyHC(s) (F, mAb S58) was inhibited. Staining with mAb F59, and thus fast MyHC expression, was also inhibited by Prdm1 knockdown (not shown). See text for quantitative analysis. Bar in A = 75 µm.