Figure 1. Comparison of basal phagocytic ability of BMDM from Fischer 344 rats and BALB/c mice.

Bone marrow derived macrophages from (A) Fischer 344 rats and (B) BALB/c mice were seeded in 6 well plates (1×10⁶ cells/well) and allowed to adhere. Cells were incubated with fluorescent microspheres (10 or 100 beads/cell) for 1 hr to allow for phagocytosis. Cells were collected into 5 ml polystyrene tubes and stained with either mouse anti-rat CD11b AF647 or rat anti-mouse CD11b APC to confirm the macrophage population. The numbers of intracelllular beads were quantified by flow cytometry (P5 = 1 bead, P6 = 2 beads, P7≥3 beads). Results are representative of two separate experiments.