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. 2010 Apr 1;5(4):e9953. doi: 10.1371/journal.pone.0009953

Figure 4. IL-7 modulates hypothalamic neuropeptide mRNA gene expression.

Figure 4

PBS ( = 5, □) or IL-7 (n = 5, ▪) was i.p. injected in 2-month-old C57BL/6 mice. The expression of hypothalamic neuropeptides was assessed by real-time PCR, in fed (A) or in re-fed (B) condition. The effects of IL-7 treatment were evaluated by calculating the relative expression levels as follows: 2ΔCt (ΔCt = mean Ct genes of interest - mean Ct GAPDH), using the raw cycle-threshold (Ct) values. *p<0.05, *** p<0.001. POMC: pro-opiomelanocortin, CART: cocaine-amphetamine related peptide, NP-Y: neuropeptide-Y, AgRP: Agouti-related peptide.