Skip to main content
. 2010 Apr 1;5(4):e9954. doi: 10.1371/journal.pone.0009954

Figure 6. Genetic rescue of DopR mutant ethanol-induced hyperactivity by restricted expression of DopR in the ellipsoid body.

Figure 6

A. Distance traveled for animals homozygous for f02676 (DopR/DopR), and heterozygous for GAL4 transgenes to drive expression of DopR in patterns that include the ellipsoid body R2/R4 neurons (11.148, 5.30), R3/R4d neurons (c232, 189Y), or the mushroom bodies (17d, 201Y). Restored expression of DopR in the R2/R4 neuron patterns but not others resulted in increased ethanol-induced hyperactivity as compared to DopR/DopR (**P<0.01, 1 way ANOVA, Dunnett's multiple comparison test, n≥10). B. Rescuing strategy results in selectively localized DopR protein expression. Confocal section of an animal homozygous for f02676 and carrying the GAL4 enhancer trap 5.30, stained for DopR protein (green) and FasII (magenta). 5.30-GAL4 drives expression of DopR protein in the ring of the ellipsoid body (eb) and the lateral triangles (ltr), but not in the mushroom bodies (mb). DopR expression in the R2 neurons is indicated by the arrowhead.