Table 1. Primer and probe sequences.

Assay	primer	oligonucleotide sequence (5′–3′)	nM	segment	detected viruses
AMsw	M+25	AgATgAgTCTTCTAACCgAggTCg	300	M	Influenza A, generic
	M-124BB	ccWgCAAARACATCYTCAAgTYTCTg	1200
	M-124sw	CTgCAAAgACACTTTCCAgTCTCTg	100
	M+64 MGB	FAM - TCAggCCCCCTCAA - MGB	100
BM	BMP-13	gAgACACAATTgCCTACCTgC	300	M	Influenza B, generic
	BMP-102AN	TTCCCACCRAACCARCARTgTAAT	1200
	BMP-72 MGB	FAM - CTgCTTTgCCTTCTC - MGB	100
H1	FluA H1 F832	ggATCAggAATCATCAMYTCAAATgC	300	HA	seasonal Influenza A(H1N1)
	FluA H1 R959	ggACACTCTCCTATTgTgACTgggTg	300
	FluA H1 MGB914	FAM - CTgCTgTTTATAgCTCC - MGB	100
H3	H3F-162	TCCTCATCAgATCCTTgATg	300	HA	seasonal Influenza A(H3N2)
	H3R-291	ACAgTTgCTgTAggCTTTgC	300
	H3S-284 MGB	FAM - CTCTATTgggRgACCC - MGB	100
H1v	FluSw H1 F236	TgggAAATCCAgAgTgTgAATCACT	300	HA	pandemic Influenza A(H1N1)v
	FluSw H1 R318	CgTTCCATTgTCTgAACTAgRTgTT	300
	FluSw H1 TM292	FAM - CCACAATgTAggACCATgAgCTTgCTgT - BHQ1	100
N1	N1P-1078 AN	AYggYAATggTgTYTggATMggRAg	1200	NA	seasonal Influenza A(H1N1)
	N1P-160 bp	ARCTYCCRCTRTAYCCHgACCARTCRgT	1200
	N1S MGB	FAM - TCCAYCCRTTRggRTCCCAAA - MGB	100
N2	N2P-P-769 AN	gATACTAAAATACTATTCATTgAggAgg	300	NA	seasonal Influenza A(H3N2)
	N2P 934 AN	ATATCTACDATgggCCTATTggAgC	300
	N2-S-840 MGB	FAM - CAYTCCTCgACATgCTg - MGB	100
N1v	FluSw N1 F1255	AgACCTTgCTTYTgggTTgAAC	300	NA	pandemic Influenza A(H1N1)v
	FluSw N1 R1334	AAggATATgCTgCTCCCRCTAgT	300
	FluSw N1 TM1310	FAM - CAgATTgTgTTCTCTTTgggTCgCCCT - BHQ1	100
FCV	FVC F54	CGTTACCGCCACACCCAT	300	n.a.	Feline Calicivirus
	FCV R141	GAGTTCACGAAAGATTTCAGACCAT	300
	FCV TM96	TexasRed - ACCCATCATTCTAACACTCCCGCCAAT - BHQ2	100

All oligonucleotide sequences are listed in 5′–3′ orientation. M = Matrix, HA = Hemagglutinin, NA = Neuraminidase, n.a. = not applicable.