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. 2010 Apr 1;5(4):e9982. doi: 10.1371/journal.pone.0009982

Figure 2. 5T4 restores CXCL12 dependent chemotaxis in differentiating 5T4KO-ES cells.

Figure 2

(A). Undifferentiated 5T4KO-ES cells forced to express 5T4 following infection with RAd-m5T4, (multiplicity of infection  = 30, dark grey columns) show no CXCL12 dependent chemotaxis comparable to undifferentiated WT-ES cells (black columns), mock (white columns), or RAd-eGFP (light grey columns) infection. (B), Differentiating 5T4KO ES cells with 5T4 expression restored by RAd-m5T4, (multiplicity of infection  = 30, dark grey columns) show CXCL12 chemotaxis comparable to differentiating WT-ES cells (black columns) but not following mock (white columns), or RAd-eGFP (light grey columns) infection. (C), Differentiating 5T4KO-ES cells chemotactic response to CXCL12 is also restored following infection with a recombinant adenovirus encoding human 5T4 (multiplicity of infection  = 30, black columns).