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. 2010 Apr 1;5(4):e9981. doi: 10.1371/journal.pone.0009981

Figure 4. Structural model and biochemical analyses of PP1c interacting sequences in DPT-AIF deletion peptides.

Figure 4

(A) Localization of the PP1c binding site in a ribbon representation deduced from the AIF crystal structure obtained from PDB. The PP1c binding site (residues 562–571 in blue) is located in an anti-parallel β-strand. The critical F566 residue mutated in the DPT-AIF2 sequence is shown in yellow. (B) DPT-AIF3 sequence and DPT-AIF4 peptides containing 8 accessible residues of AIF564–571. (C) Pull down experiments to analyze co-precipitation of DPT-AIF3 and DPT-AIF4 peptides with PP1c for HeLa cell extracts.