Table 3.

List of primers used in this study.

Primer	Primer Sequence
NP	5-AATG(A/C)T(G/T)(A/T)(C/T)GAACGGCCT(C/T)A-3
NR	5-TTAAGC(G/A)(G/T)(A/T/C)(G/T)CC(G/A)TTGTAG-3
P246	5-AA(G/A)TA(C/T)AT(C/T)GTCGTC(C/T)(A/T)(C/G)AAG-3
1480TSP1	5-ATGAGAGATGCGGTCAAGGC-3
1480TSP2	5-CGACTTCAGGCGAGTTCAGAATC-3
1480TSP3	5-CAAAGCCGCCAGTGTATCCAG-3
22TSP1	5-TGAGAGATGCGGTCAAGAC-3
22TSP2	5-TATCCTGAAGAGTAGCAGCGTCG-3
22TSP3	5-GGCGTGGAGAGATGAAATGCG-3

NP and NR were used to amplify the full-length encoding genes of subtilisin-like serine proteases from A. musiformis and A. yunnanensis. P246 and NR were used to amplify the 3' fragment of the subtilisin-like serine protease genes from M. coelobrochum, M. psychrophilum and D. shizishanna. 1480TSPN (N = 1, 2, 3) and 22TSPN (N = 1, 2, 3) were used to amplify the 5' fragment sequences from M. coelobrochum and D. shizishanna by using the DNA Walking Speedup™ PreMix Kit (Seegene, Korea), respectively