Table 2.
Intra- and interday variability for the assay of the three Panax notoginseng saponins in beagle dog plasma (n = 6)
|
Spiked conc. (ng/mL) |
Intraday | Interday | ||||
|---|---|---|---|---|---|---|
| Measured conc. (ng/mL) | RSD (%)a | Accuracy (%)b | Measured conc. (ng/mL) | RSD (%) | Accuracy (%) | |
| Notoginsenoside R1 | ||||||
| 2.8 | 2.8 | 1.27 | 100.0 | 2.68 | 1.49 | 95.71 |
| 14 | 13.9 | 0.76 | 99.29 | 12.89 | 9.62 | 92.1 |
| 140 | 148 | 8.22 | 105.7 | 131.67 | 10.11 | 105.7 |
| Ginsenoside Rg1 | ||||||
| 3.8 | 3.6 | 5.13 | 94.7 | 3.96 | 11.96 | 104.2 |
| 19 | 17.6 | 3.65 | 92.6 | 18.3 | 8.95 | 96.3 |
| 190 | 197.3 | 6.59 | 103.8 | 193.7 | 7.66 | 101.7 |
| Ginsenoside Rb1 | ||||||
| 18 | 18.1 | 3.07 | 100.6 | 17.6 | 5.36 | 96.11 |
| 180 | 165.3 | 2.73 | 91.83 | 179.7 | 5.05 | 99.50 |
| 900 | 912.67 | 3.90 | 101.3 | 871.3 | 2.38 | 96.87 |
Note: aRelative standard deviation; bAccuracy (%) = [1--(nominal concentration -- mean of measured concentration)/nominal concentration] × 100