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Undefined cluster distribution in a de novo NGS generated SNP array (SAM). Failure to define separate genotype clusters for newly identified variants TD102 (A) and TD108 (B) was due to incorrect sequence input into the GoldeGate assay design software. The failure of one ASO to bind resulted in the different genotype groups being separated by a difference in fluorescence intensity of one allele along the Y-axis of the cluster plot.
