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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: J Med Chem. 2010 Apr 8;53(7):2757–2765. doi: 10.1021/jm901757t

Figure 4.

Figure 4

A. Western blot showing expression of endogenous BCA2 in T47D and the isogenic MDA-MB-231 parental and MDA-MB-231/ER breast cancer cell lines. Anti-BCA2 antibodies were developed by us and used as previously described.7 Beta-actin was used as equal loading control. B. Western blot analysis of BCA2 autoubiquitination in the presence of disulfide analogs at 5 and 50 μM of drug dissolved in dimethylsulfoxide. The membrane was probed with anti-ubiquitin antibodies. Shown are the ubiquitin signal (8kD) and high molecular weight polyubiquitinylated BCA2 (>98 kD). Polyubiquitinated proteins are typified by a high molecular weight smear.