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Invasion of epithelial cells by SPI1-induced bacteria. To directly compare the invasiveness of aer-LL and μaer-ST S. Typhimurium, HeLa cells were infected for 10 min at 37 °C (m.o.i. 50–60). At 1 h p.i., cells were lysed and intracellular bacteria were enumerated by plating. The means±sd from three independent experiments are shown. (a) Invasion expressed as c.f.u. per well. (b) Invasion expressed as % of inoculum. In both cases P<0.001, Student's t-test.
