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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Prostate. 2010 May 1;70(6):675–688. doi: 10.1002/pros.21112

Figure 1. EZH2 expression, overexpression, and knockdown in prostate cancer cell lines.

Figure 1

A. Schematic diagrams of EZH2-overexpressing and GFP control lentiviruses. LTR: Long Terminal Repeat; cppt: central polypurine tract; CMV: human cytomegalovirus promoter; IRES: ECMV internal ribosomal entry site; EGFP; enhanced green fluorescent protein; wpre: woodchuck post-transcriptional regulatory element. B. Schematic diagrams of short hairpin lentiviral vectors specific against EZH2 and Firefly Luciferase (control). U6: U6 pol III promoter; PGK: Phosphoglycerate Kinase Promoter. C. qRT-PCR examination of endogenous levels of EZH2 and EED in a panel of prostate cell lines, and western blot examination of endogenous EZH2. 22Rv1, DU145, LAPC4, LNCaP, and PC3 are prostate cancer cell lines. BPH1 is a prostatic epithelial cell line. α-EZH2: antibody specific for endogenous and exogenous EZH2; α-Actin: antibody specific for β-Actin, used as a loading control for all western blots. D. Western Blot examination of shRNA mediated knockdown of HA-EZH2 in 293T cells.