Table I. Quantification of Nuclear Staining Intensity following Overexpression or Knockdown of EZH2.

	Endogenous*			Expression**		Nuclear Staining**
	EZH2	EED	Ratio†	EZH2	shEZH2	EZH2 ± SD	shEZH2 ± SD
LAPC4	0.060	0.101	0.6	11.0	0.3	1.52 ± 0.35	0.53 ± 0.02
LNCaP	0.032	0.027	1.2	4.3	0.5	1.8 ± 0.04	0.45 ± 0.01
22Rv1	0.066	0.036	1.8	7.0	0.4	1.46 ± 0.14	0.31 ± 0.03
PC3	0.038	0.018	2.1	9.0	0.3	1.35 ± 0.20	0.51 ± 0.15
DU145	0.026	0.047	0.6	5.6	0.8	1.86 ± 0.04	0.69 ± 0.13

EZH2: Overexpression of EZH2, shEZH2: Knockdown of EZH2.

^*Relative to β-Actin;

^**Nuclear staining for H3K27Me3 was quantified in at least two GFP positive cells in each field, quantities shown are fold change over respective controls;

^†Ratio of EZH2 to EED.