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Characterization of human EVT cells. Reverse transcriptase-PCR analysis of (A) IL11RA, (B) IL6ST mRNA, and (C) 18S mRNA expression (loading control). −, Negative controls. Immunoreactive IL11RA protein in (D) HTR-8/SVneo and (E) primary EVTs (insets, negative controls). F) Interleukin 11 secretion by EVT cells (mean ± SEM). Data are shown for representatives of four independent experiments in each case. Bars = 50 μm.
