Expression of slits and robos in the statoacoustic ganglion (SAG) determined by in situ hybridization on frozen sections in either the horizontal (A–G) or transverse plane (H–I). Section H is double labeled with a neurofilament antibody. A,B: Robo2 expression in the SAG at stages 19 (A) and 21 (B). C,D: Stage 23 serial sections labeled with robo2 (C) and slit2 (D). E–G: Stage 23 serial sections labeled with slit2 (E) or double labeled with HuC/D and Islet1 antibodies (F,G respectively). H,I: slit1 expression in the auditory (H), vestibular (I), and geniculate ganglia (I) at stage 26. Scale Bars, 100µm. Orientation: A, anterior; D, dorsal; M, medial. Abbreviations: AG, auditory ganglion; GG, geniculate ganglion; HB, hindbrain; VG, vestibular ganglion.