Table 3.

Maternal cigarette smoking significantly alters the relative ratios of active LHCGR isoforms but not expression levels (mean ± sem)

Isoform	Control	Smoker	P value
n	12	10
Expression levels
Complete exons 9-11 (a)	2.84 ± 1.42	5.64 ± 1.61	0.813
Complete 6A (a)	1.04 ± 0.36	1.84 ± 0.41	0.921
Terminal 6A (i)	2.87 ± 0.58	2.66 ± 0.66	0.651
Complete 6A short (i)	0.95 ± 0.21	1.14 ± 0.24	0.911
Complete 6A long (i)	0.16 ± 0.05	0.04 ± 0.05	0.775
Expression ratios
Complete, 6A (a)	1.00 ± 0.02	1.00 ± 0.25	0.078
Terminal 6A (i)	2.76 ± 0.46	1.45 ± 0.52	0.013
Complete 6A short (i)	0.91 ± 0.16	0.62 ± 0.18	0.167
Complete 6A long (i)	0.15 ± 0.04	0.02 ± 0.05	0.735

	Control r	P value	Smoker r	P value
Relationship to gestational age (wk)
Complete exons 9-11 (a)	0.900	<0.001	0.571	0.085
Complete 6A (a)	0.730	0.007	0.571	0.085
Terminal 6A (i)	0.825	0.001	0.568	0.087
Complete 6A short (i)	0.673	0.017	0.526	0.118
Complete 6A long (i)	0.515	0.087	0.228	0.524

Active LHCGR isoforms are indicated by a in parentheses, inactive by i in parentheses. Complete exons 9-11 is the sequence used in (11,13). Transcript values were divided by complete 6A values to obtain the isoform ratios. Significantly different P values are highlighted in bold.