Two different isolates of fetal mesenchymal stem cells differentiate into adipocytes which resemble white and brown adipocytes respectively.
A) UCP-1 mRNA expression in fMSC 10+1 and 9+4 isolates after 21 days of differentiation.
B) UCP-1 mRNA expression in fMSC 9+4 isolatre before and after 21 days of differentiation, and differentiated human adult sub-cutaneous adipocytes (hPA). nd = not detected.
C and D) mRNA expression of PGC1α and RIP140 (C) and PRDM16 (D) in the two isolates before and after differentiation. ** indicates p<0.001, * p<0.05 for comparison between the two isolates.
E) Expression of UCP-1 mRNA in 9+4 isolate after 21 days differentiation, before and after treatment with 10μM Isoproteranol for 5 hours.
Error bars indicate SD. nd indicates not detected. Relative expression in arbitrary units.