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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cell Res. 2010 Jan 26;20(4):434–444. doi: 10.1038/cr.2010.11

Fig 4.

Fig 4

UCP-1 expression in fMSC 9+4 adipocytes can be induced by Rosiglitazone treatment, and reduced by the ERRα inverse agonist.

mRNA expression determined by qPCR

A)UCP-1 expression following Rosiglitazone or XCT790 treatment for 48 hours. * p=<0.05, ** = p<0.005.

B)UCP-1 expression after 48 hour treatment with PPARg anatagonist G3335 * = p<0.05.

C) Chromatin Immunoprecipitation showing ERRa bound to the UCP1 enhancer element in differentiated 9+4 fMSCs, but not to a region in the coding sequence of the same gene.

D) Reporter assay using this construct in Cos-1 cells. ERRa activates the enhancer, and its activity is further trans-activated by PGC1a and repressed by RIP140. The ERRa inverse agonist XCT790 prevents this induction.

E) ERRα expression in 10+1 and 9+4 isolates before and after 21 days adipogenic differentiation. mRNA expression by qPCR. Error Bars indicate SD.