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. 2010 Apr 2;5(4):e9994. doi: 10.1371/journal.pone.0009994

Figure 1. Both 15aa and 20aa repeat regions of APC interact with topo IIα.

Figure 1

(A) Schematic diagram of APC protein showing location of M2 and M3-APC fused to the C-terminus of EGFP and used in all cell studies. (B) A GFP antibody was used to immunoprecipitate (IP) EGFP from HCT116βw cells expressing either EGFP-M2-APC, EGFP-M3-APC or EGFP. Immunoblots (IB) were probed using antibodies indicated to the left of the gel. Ten percent input equals 10 µg total protein. Topo IIα co-precipitates with both M2-APC and M3-APC, but not with EGFP. Topo IIβ does not co-precipitate with M2- or M3-APC, but β-catenin does co-precipitate with both. Blots probed for α-tubulin served as a loading control for the input samples and a negative control for the immunoprecipitations. (*) marks migration of antibody heavy chain. Representative blots from three independent experiments are shown.