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. 2010 Apr 2;5(4):e9994. doi: 10.1371/journal.pone.0009994

Figure 3. Cells expressing M2- or M3-APC progressively accumulate in G2.

Figure 3

(A) Histograms showing representative FACS displays of cell cycle distribution assessed by Hoechst blue staining at 24, 48, and 72 hours post-transfection with expression constructs for EGFP fused M2- or M3-APC, or EGFP alone. Only EGFP-positive cells are displayed. (B) Bar graphs show FACS-based cell cycle distribution at 24, 48, and 72 hours post-transfection as the average of three independent experiments. Error bars represent standard deviation. When compared to cells expressing only EGFP, by 72 hours post-transfection, the fraction of M2-APC expressing cells in G2/M increased by 2.4-fold, and the S phase decreased by 77%; the fraction of M3-APC expressing cells in G2/M increased by 2-fold, and the S phase decreased by 31%. (C) Live cell scoring for mitotic indices of 100 EGFP-positive cells 24 hours and 48 hours post-transfection. Three independent experiments revealed no change in the mitotic population when cells expressed M2 (24 hours, 2±1, p = 0.74; 48 hours, 3±3, p = 0.19) or M3-APC (24 hrs, 6±3, p = 0.67; 48 hrs, 1±1, p = 0.29), as compared to cells expressing EGFP alone (24 hours, 3±3; 48 hours 4±2).