|
Bacterial strains
|
|
E. coli DH5α |
F- endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 deoR
|
TransGen |
|
Sphingomonas sp. XLDN2-5 |
Aerobic, rod shaped, degrades carbazole |
Lab stock |
|
Plasmids
|
| pUC19 |
Ampr
lacZ, pMB9 replicon, M13IG |
TaKaRa |
| pMD18-T |
Clone vector |
TaKaRa |
| pEASY-Blunt |
Clone vector |
TransGen |
| pBY13 |
Ampr; pUC19 with 7.8-kb fragment that hybridized with car probe |
This study |
| p6100-a |
Ampr; pMD18-T with 1-kb fragment obtained by genome walking |
This study |
| p6100-1 |
Ampr; pMD18-T with 4.3-kb fragment obtained by IS-based PCR using pIS6100-F1 and pIS6100-R1 |
This study |
| p6100-2 |
Ampr; pMD18-T with 2.8-kb fragment obtained by IS-based PCR using pIS6100-F1 and pIS6100-R1 |
This study |
| P6100-3 |
Ampr; pMD18-T with 5.2-kb fragment obtained by IS-based PCR using pIS6100-R1 |
This study |
| pUcarAa |
Ampr; pUC19 with 1.2-kb SphI-XbaI fragment containing the carAa gene of strainXLDN2-5 |
This study |
| pUcarAc |
Ampr; pUC19 with 0.3-kb XbaI-KpnI fragment containing the carAc gene of strain XLDN2-5 |
This study |
| pUfdr |
Ampr; pUC19 with 1.3-kb KpnI-EcoRI fragment containing the fdr gene of strain XLDN2-5 |
This study |
| pEcarAaAc |
Ampr; Kar, pEASY-Blunt with 1.5-kb fragment containing the carAa and carAc genes of strain XLDN2-5 |
This study |
| pUcarAaAc |
Ampr; 1.5-kb SphI-KpnI fragment containing carAaAc from pUcarAaAc cloned into SphI-KpnI site of pUC19 |
This study |
| pUcarAaAcfdr |
Ampr; 1.5-kb SphI-KpnI fragment containing carAaAc from pUcarAaAc cloned into SphI-KpnI site of pUfdr |
This study |
| pUantAcdb |
Ampr; pUC19 with 2.1-kb HindIII-EcoRI fragment containing the antAcAdAb genes of strainXLDN2-5 |
This study |
| pUantAcdba |
Ampr; pUC19 with 3.5-kb HindIII-EcoRI fragment containing the antAcAdAbAa genes of strainXLDN2-5 |
This study |
