Table 1.
Microarray analysis of monocytes treated with R-848. PBM from 3 different donors were incubated overnight with or without 1 µM R-848 and then subjected to Affymetrix microarray analysis. Transcripts with fold-increases of 2 or greater, with average log2 expression of 3 or greater and with ontologies of “immune response” or “inflammatory” were selected. Highlighted in gray are the activating FcγR.
| Symbol | FC | p val | Description | Symbol | FC | p val | Description |
|---|---|---|---|---|---|---|---|
| ADA | 4.317346 | 1.4E-05 | adenosine deaminase | IL1RN | 25.6737 | 4.5E-07 | interleukin 1 receptor antagonist |
| ADORA2A | 40.55086 | 8.8E-12 | adenosine A2a receptor | IL2RA | 43.53276 | 1.6E-07 | interleukin 2 receptor, alpha |
| ADRB2 | 16.73249 | 8.7E-06 | adrenergic, beta-2-, receptor, surface | IL2RG | 2.428115 | 0.004 | interleukin 2 receptor, gamma |
| AGER | 3.71607 | 9.8E-05 | adv glycosylation end product-specific receptor | IL4R | 13.91337 | 1.3E-06 | Interleukin 4 receptor |
| APOL3 | 2.290186 | 0.045 | apolipoprotein L, 3 | IL6 | 489.0295 | 5.4E-09 | interleukin 6 (interferon, beta 2) |
| AQP9 | 5.560471 | 1.2E-08 | aquaporin 9 | IL6ST | 3.797162 | 0.001 | interleukin 6 signal transducer |
| B4GALT1 | 7.023606 | 1.5E-06 | beta 1,4- galactosyltransferase, polypeptide 1 | IL7R | 45.28874 | 1.8E-07 | interleukin 7 receptor |
| BCL3 | 2.973849 | 1.4E-06 | B-cell CLL/lymphoma 3 | IL8 | 3.7873 | 0.005 | interleukin 8 |
| BCL6 | 4.485563 | 2.8E-11 | B-cell CLL/lymphoma 6 | IRAK2 | 25.49053 | 3.2E-08 | interleukin-1 receptor-associated kinase 2 |
| CCL18 | 8.512269 | 4.8E-06 | chemokine (C-C motif) ligand 18 | ITCH | 6.845941 | 0.005 | itchy homolog E3 ubiquitin protein ligase |
| CCL19 | 190.8174 | 4.1E-09 | chemokine (C-C motif) ligand 19 | ITGAL | 3.649082 | 6.4E-05 | integrin, alpha L (antigen CD11A (p180) |
| CCL2 | 6.25498 | 0.024 | chemokine (C-C motif) ligand 2 | LAIR1 | 7.521857 | 2.5E-06 | leukocyte-associated Ig-like receptor 1 |
| CCL20 | 55.74429 | 3.0E-07 | chemokine (C-C motif) ligand 20 | LCP2 | 2.979506 | 3.8E-04 | lymphocyte cytosolic protein 2 |
| CCL3 | 20.34984 | 1.4E-06 | chemokine (C-C motif) ligand 3 | LILRA1 | 27.92517 | 3.2E-12 | leukocyte immunoglobulin-like receptor, A1 |
| CCL4 | 18.7361 | 1.6E-06 | chemokine (C-C motif) ligand 4 | LILRA2 | 2.971885 | 0.003 | leukocyte immunoglobulin-like receptor, A2 |
| CCL7 | 4.251229 | 0.040 | chemokine (C-C motif) ligand 7 | LILRA3 | 35.13229 | 2.9E-09 | leukocyte immunoglobulin-like receptor, A3 |
| CD163 | 28.96202 | 1.2E-06 | CD163 molecule | LILRB1 | 6.353475 | 7.9E-08 | Leukocyte immunoglobulin-like receptor, B1 |
| CD274 | 31.7408 | 3.6E-09 | CD274 molecule | LILRB2 | 7.090295 | 7.9E-08 | leukocyte immunoglobulin-like receptor, B2 |
| CD40 | 3.132347 | 0.028 | CD40 molecule | LILRB3 | 3.073823 | 1.5E-06 | leukocyte immunoglobulin-like receptor, B3 |
| CD55 | 4.733903 | 1.3E-06 | CD55 molecule | LILRB4 | 2.554202 | 0.016 | leukocyte immunoglobulin-like receptor, B4 |
| CD59 | 19.06185 | 2.9E-09 | CD59 molecule | LOC65387 | 3.415538 | 0.030 | similar to Complement C3 precursor |
| CD80 | 18.26656 | 1.9E-05 | CD80 molecule | MEFV | 14.03661 | 0.001 | Mediterranean fever |
| CD83 | 2.445838 | 0.003 | CD83 molecule | MGLL | 2.640648 | 0.023 | monoglyceride lipase |
| CFB | 59.44535 | 1.4E-08 | complement factor B | MS4A1 | 3.285111 | 0.006 | Membrane-spanning 4-domains, A1 |
| CHST2 | 4.048418 | 0.001 | carbohydrate sulfotransferase 2 | NFE2L1 | 3.47481 | 1.3E-04 | nuclear factor (erythroid-derived 2)-like 1 |
| CLEC4D | 5.729237 | 3.9E-05 | C-type lectin domain family 4, member D | NLRP3 | 3.529458 | 0.026 | NLR family, pyrin domain containing 3 |
| CLEC4E | 2.414824 | 0.020 | C-type lectin domain family 4, member E | NR3C1 | 4.879714 | 3.8E-08 | nuclear receptor subfamily 3, C1 |
| CLU | 2.289794 | 0.021 | clusterin | PAG1 | 13.00331 | 2.6E-08 | p-protein assoc w/ glycosphingolipid microdomains 1 |
| COL4A3BP | 2.603515 | 4.2E-04 | collagen, type IV, alpha 3 binding protein | PDCD1LG | 7.568735 | 7.5E-06 | programmed cell death 1 ligand 2 |
| CSF3 | 36.21415 | 2.0E-07 | colony stimulating factor 3 | POMP | 2.532659 | 3.7E-04 | proteasome maturation protein |
| CXCL1 | 97.88367 | 1.2E-12 | chemokine (C-X-C motif) ligand 1 | POU2F2 | 2.35123 | 4.9E-05 | POU class 2 homeobox 2 |
| CXCL13 | 116.9714 | 2.0E-09 | chemokine (C-X-C motif) ligand 13 | PRELID1 | 3.945781 | 1.3E-05 | PRELI domain containing 1 |
| CXCL2 | 8.593548 | 1.1E-05 | Chemokine (C-X-C motif) ligand 2 | PRG2 | 3.749564 | 4.8E-04 | proteoglycan 2, bone marrow |
| CXCL3 | 17.77199 | 7.6E-07 | chemokine (C-X-C motif) ligand 3 | PRKCA | 3.283269 | 0.010 | protein kinase C, alpha |
| CXCL5 | 15.40997 | 6.8E-05 | chemokine (C-X-C motif) ligand 5 | PTGS2 | 121.221 | 2.5E-10 | prostaglandin-endoperoxide synthase 2 |
| CYBB | 3.719852 | 0.002 | Cytochrome b-245, beta polypeptide | PTX3 | 34.45957 | 6.8E-14 | pentraxin-related, rapidly induced by IL-1 beta |
| DPP8 | 3.208713 | 0.021 | dipeptidyl-peptidase 8 | RAC1 | 3.784847 | 0.005 | Ras-related C3 botulinum toxin substrate 1 |
| EBI3 | 25.25581 | 9.2E-07 | Epstein-Barr virus induced gene 3 | RELA | 11.36527 | 4.4E-04 | V-rel homolog A, p65 |
| EREG | 4.980403 | 3.3E-04 | epiregulin | RIPK2 | 2.190812 | 0.034 | receptor-interacting serine-threonine kinase 2 |
| ETS1 | 13.56834 | 4.3E-04 | v-ets E26 homolog 1 | RNF19B | 10.31492 | 6.0E-05 | ring finger protein 19B |
| F3 | 18.19057 | 5.3E-06 | coagulation factor III | S100A12 | 3.133215 | 0.014 | S100 calcium binding protein A12 |
| FCER1G | 2.391636 | 0.001 | Fc fragment of IgE, gamma polypeptide | S100A8 | 15.18746 | 2.1E-04 | S100 calcium binding protein A8 |
| FCGR1A | 21.46627 | 1.8E-07 | Fc fragment of IgG, high affinity Ia, receptor (CD64) | S100A9 | 3.26703 | 0.006 | S100 calcium binding protein A9 |
| FCGR1B | 3.287157 | 0.042 | Fc fragment of IgG, high affinity Ib, receptor (CD64) | SEMA3C | 22.85418 | 1.0E-11 | sema, immunoglobulin, short basic, secreted, 3C |
| FCGR2A | 2.545392 | 0.001 | Fc fragment of IgG, low affinity IIa, receptor (CD32) | SLC11A1 | 10.12225 | 7.2E-08 | solute carrier family 11, member 1 |
| FOXP1 | 3.44095 | 3.0E-06 | forkhead box P1 | SNF1LK | 7.138636 | 0.001 | SNF1-like kinase |
| FPR1 | 3.575507 | 3.0E-04 | formyl peptide receptor 1 | SPN | 3.129464 | 0.027 | sialophorin (leukosialin, CD43) |
| FPRL1 | 34.54009 | 5.9E-09 | formyl peptide receptor-like 1 | TARP | 11.3274 | 0.011 | T cell receptor gamma constant 2 |
| FUS | 6.003565 | 0.016 | Fusion (in t(12;16) in malignant liposarcoma) | TBK1 | 2.765583 | 3.2E-05 | TANK-binding kinase 1 |
| HAMP | 59.32565 | 1.4E-12 | hepcidin antimicrobial peptide | TGM2 | 16.02947 | 4.5E-08 | Transglutaminase 2 |
| HDAC7A | 2.141959 | 0.045 | histone deacetylase 7A | TLR1 | 2.055846 | 0.005 | toll-like receptor 1 |
| HRH1 | 10.03894 | 7.2E-06 | histamine receptor H1 | TLR4 | 2.422299 | 0.033 | toll-like receptor 4 |
| HSPC111 | 6.28524 | 0.008 | hypothetical protein HSPC111 | TNF | 12.9597 | 3.4E-07 | tumor necrosis factor, member 2 |
| IFNG | 26.61823 | 1.4E-04 | interferon, gamma | TNFAIP1 | 2.2312 | 1.9E-04 | tumor necrosis factor, alpha-induced protein 1 |
| IL10 | 39.7384 | 3.3E-08 | interleukin 10 | TNFAIP6 | 14.66536 | 6.8E-07 | tumor necrosis factor, alpha-induced protein 6 |
| IL12B | 3.38804 | 2.6E-04 | interleukin 12B, p40 | TNFRSF9 | 2.658242 | 0.020 | tumor necrosis factor receptor, member 9 |
| IL1A | 61.94209 | 8.4E-08 | interleukin 1, alpha | TNFSF9 | 3.521049 | 8.9E-05 | tumor necrosis factor, member 9 |
| IL1B | 26.07393 | 7.7E-09 | interleukin 1, beta | TREM1 | 6.539373 | 6.1E-06 | triggering receptor expressed on myeloid cells 1 |
| IL1F9 | 18.53308 | 3.7E-05 | interleukin 1 family, member 9 | VAV1 | 7.233896 | 1.8E-07 | vav 1 guanine nucleotide exchange factor |
| IL1RAP | 3.382441 | 1.2E-04 | interleukin 1 receptor accessory protein |