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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Brain Res. 2010 Feb 12;1325C:128–140. doi: 10.1016/j.brainres.2010.02.019

Figure 3. Verification of microarray data with quantitative real-time PCR and immunoblotting.

Figure 3

A. Quantitative real-time PCR analysis of gene expression in the cerebellum of Npc1-/- mice compared with Npc1+/+ mice. Shown are means + SEM for mRNA levels relative to that of Npc1+/+ mice (values >1 indicate increased expression while those <1 represent decreased expression). N=4 for each group. * p<0.05, ** p<0.01. GAPDH mRNA was used as endogenous control. B. Immunoblots of ApoE and GAPDH (used as loading control) with samples from cerebellum of Npc1+/+ and Npc1-/- mice confirmed that ApoE levels were significantly increased in cerebellum from Npc1-/- mice (n=4, p <0.05).