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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cancer Res. 2010 Mar 16;70(7):2819–2828. doi: 10.1158/0008-5472.CAN-09-1915

Figure 1. Tie2 signaling directly regulates osteoclastogenesis in vitro.

Figure 1

TL1 ES cells and Tie2 null ES cells were induced differentiation toward osteoclast for approximately 3 weeks in vitro. TRAP staining was performed to detect matured multinuclear osteoclasts as marked by arrows (Panel A). Number of TRAP positive multinuclear osteoclasts were counted in randomly selected field under microscopy and graphed between wild type and Tie2 null ES cells (Panel B). The experiment was done in triplicate, and repeated three times. Results are mean ± SE. *p<0.05 calculated with Statview 5. TL1 ES cells were induced differentiation toward osteoclast for approximately 3 weeks in the presence of recombinant ExTek at 100ng/ml or control protein. The media were replaced twice a week. TRAP staining was applied to detect matured osteoclasts as pointed by arrows (Panel C). Number of TRAP positive cells were counted in randomly selected field under microscopy and graphed between the two groups (Panel D). The experiment was done in triplicate, and repeated three times. Results are mean ± SE. * p<0.05 calculated with Statview 5.