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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cancer Res. 2010 Mar 16;70(7):2819–2828. doi: 10.1158/0008-5472.CAN-09-1915

Figure 3. Genetic deletion of Tie2 had no effect on osteoblastogenesis in vitro.

Figure 3

TL1 ES cells and Tie2 null ES cells were induced differentiation toward osteoblast for approximately 4 weeks in vitro. At the end of the experiments, cells were stained with Alizarin Red for osteoblasts (Panel A). Number of Alizarin Red positive cells were counted and graphed between wild type and Tie2 null ES cells (Panel B). Results are mean ± S.E. from three separate experiments. TL1 ES cells were induced to differentiate toward osteoblast in the presence of recombinant ExTek at 100ng/ml or control BSA protein (Panel C). Number of Alizarin Red positive cells were counted and graphed between the two groups (Panel D). Results are mean ± S.E. from three separate experiments.