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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cancer Res. 2010 Mar 23;70(7):2891–2900. doi: 10.1158/0008-5472.CAN-09-3139

Figure 3.

Figure 3

Inducible expression of Foxd3 blocks melanoma growth in vitro. A, WM793TR cells expressing doxycycline-inducible β-galactosidase (LacZ) or murine Foxd3 were induced with 0.1 μg/ml doxycycline for 5 days. Lysates were immunoblotted for the respective transgenes, ERK1/2 and the C-terminal V5-epitope tag found on both transgenes. B, Induced WM793TR cells were stained with anti-V5-epitope (green) to detect LacZ or Foxd3, as well as Hoechst 33342 (blue) and anti-RhoGDI (red) to label nuclei and cytoplasm, respectively. C, WM793TR and WM115TR cells expressing LacZ or Foxd3 were treated −/+ doxycycline, trypsinized, counted and replated every 3 days for nine days. Average cell numbers and standard error bars are given for 3 independent experiments. * p<0.05. D, SK-MEL-28TR and A375TR cells expressing LacZ or Foxd3 were embedded in 0.3% agar and allowed to grow for approximately 2 weeks. SK-MEL-28TR colonies were photographed at 20X magnification and A375TR colonies were photographed at 10X magnification.