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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cancer Res. 2010 Mar 23;70(7):2891–2900. doi: 10.1158/0008-5472.CAN-09-3139

Figure 5.

Figure 5

Expression of Foxd3 up-regulates p21Cip1 in melanoma cell lines but not in a breast or a fibrosarcoma cell line. A, Foxd3-inducible WM115, SK-MEL-28, and A375 cells were treated with 0.1 μg/ml doxycycline for 5 days. Cell lysates were analyzed by Western blotting for the V5-epitope, cyclin A, p21Cip1 and ERK1/2. B, Foxd3 and LacZ inducible forms of the human breast cancer line, MDA-MB-231, and human fibrosarcoma line, HT-1080, were generated. Cell lines were treated −/+ 0.1 μg/ml doxycycline for 5 days. Cell lysates were analyzed by Western blotting for the V5-epitope, p21Cip1 and ERK1/2. C, WM793 expressing inducible LacZ, mouse Foxd3-V5, human FOXD3 and human FOXD3-V5 were treated −/+ doxycycline for 5 days. Lysates were analyzed by Western blotting for FOXD3, p21Cip1, cyclin A, phospho-ERK1/2 (pERK1/2) and total ERK1/2.