Figure 5.

Depolarization-induced pCREB is decreased in inflamed colon. (A) Nuclear extracts were prepared from distal colon of normal (Nor) and inflamed (Inf) mice treated with 80 mM KCl, and expression of pCREB was measured by immunoblots with anti-pCREB and CREB antibodies. Phospho-CREB expression was not enhanced by depolarization in inflamed colon. **P < 0.01 versus Nor + KCl (n= 3). (B) quantitative comparison of gene expression shows increased expression of IL-1β during colonic inflammation. Total RNA was obtained from normal and inflamed distal colon tissues, and fold increase of IL-1β mRNA level was assessed by real-time RT-PCR after being normalized by the mRNA level of 18S. IL-1β mRNA was increased by 2.5-fold due to inflammation. All real-time PCR experiments were carried out in triplicate. Data analysed by unpaired t-test; n= 4. **P < 0.01 versus normal. CREB, cyclic AMP response element binding protein.