Figure 1.
ER binding competition for nebivolol in N2A cells. In this assay, 2 × 10−9 M [3H]-E2 was competing for binding to the ER with increasing concentrations of nebivolol from 1 × 10−6 M to 2 × 10−2 M. A 100-fold molar excess of unlabelled E2 was used to determine the specific displaceable binding. Specific binding was calculated based on the percentage of remaining isotopic activity. Results are expressed as the mean of ±SEM of three independent measurements. The nebivolol concentration at 50% inhibition of [3H]-E2 binding (IC50) = 0.8 × 10−5 M.