Table 1.

PCR primers and conditions

Gene	Symbol	Species	GenBank accession no.	Primer sequence	Annealing temp. (°C)Elongation time (s)Product size (bp)
Androgen receptor	AR	Human	BC132975	5′- gtcaactccaggatgctctac-3′	62, 25, 321
		Mouse	M37890	5′-ccaggagcttggtgagctggta-3′
ADAM metallopeptidase domain 9	ADAM 9	Human	BC126406	5′- cagaatggatatccttgccag-3′	62, 40, 496
		Mouse	BC047156	5′- tcacagtgacaattcttattgc-3′
ADAM metallopeptidase domain 10	ADAM 10	Human	BC066207	5′- ggaacacgagaagctgtgattg-3′	62, 40, 229
		Mouse	AF009615	5′- cagtagtcatcatgattctgctc-3′
ADAM metallopeptidase domain 17	ADAM 17	Human	BC146658	5′- ggattcctttcagcattcttgtc-3′	62, 40, 260
		Mouse	BC136783	5′- gtgagtctgtgctggggtcttc-3′
Beta-site APP-cleaving enzyme 1	BACE 1	Human	NM_012104	5′-cgcagacgctcaacatcc-3′	62, 35, 139
				5′- gggcacatacacacccttcc-3′
		Mouse	NM_011792	5′- cagtgggaccaccaaccttc-3′	60, 30, 70
				5′-gctgccttgatggacttgac-3′
Baculoviral IAP repeat-containing 3	Birc3	Human	AF070674	5′-tactacataggacctggaga-3′	62, 30, 383
		Mouse	NM_007464	5′-caagtactcacaccttggaaac-3′
Heat shock 27 kDa protein 1	Hsp B1	Human	NM_001540	5′-ggacgagctgacggtcaag-3′	60 30, 245
				5′-cgcgactcgaaggtgactg-3′
		Mouse	L27560	5′-atcccctgagggcacactta-3′	58, 35 137
				5′-ccagactgttcagagttcccag-3′
Trefoilfactor 1	pS2	Human	NM_003225	5′-acaaggtgatctgcg-3′	58, 35 190
				5′-gaagcaccaggggac-3′
		Mouse	NM_009362	5′-cactcgtggtcttcccgtga-3′	60, 40, 411
				5′-ctgtgtcaccagccagatcca-3′
Presenilin 1	PSEN1	Human	NM_000021	5′-ctctgcaacagtgtcttgtg-3′	62, 30 400
				5′-gttgctgtggactacattac-3′
		Mouse	NM_008943	5′-acaatggtgtggttggtgaat-3′	62, 30 360
				5′-agcaggctatggttgtgttcc-3′
Progesterone receptor	PGR	Human	M15716	5′-acaccttgcctgaagtttcg-3′	62, 20, 195
				5′-ctgtccttttctgggggact-3′
		Mouse	M68915	5′-gattcagaagccagccagagc-3′	62, 20 267
				5′-gacctccaaggaccatgccagc-3′
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Human	NM_002046	5′-cctgcaccaccaactgcttagc-3′	62 30 334
		Mouse	NM_008084	5′-caccaccttcttgatgtcatc-3′
Ribosomal protein L19	RPL19	Human	NM_000981	5′-gaaatcgccaatgccaactc-3′	62, 30, 413
		Mouse	NM_009078	5′-ttccttggtcttagacctgcg-3′