Table 3.
Interactions of flavonoids and metabolites with the platelet receptor ligands, collagen and fibrinogen
| Flavonoid/metabolite |
KD (kd/ka)±SEM (M) |
|
|---|---|---|
| Collagen | Fibrinogen | |
| Quercetin | 3.62 ± 3.55 (×10−6) | 9.96 × 10−6± 5.18 (×10−6) |
| Tamarixetin | 1.06 ± 0.52 (×10−6) | 6.15 ± 3.64 (×10−10) |
| Apigenin | 3.38 ± 2.61 (×10−7) | 2.99 ± 1.39 (×10−7) |
| Catechin | 4.81 ± 2.43 (×10−9) | 5.52 ± 1.26 (×10−7) |
Flavonoid and metabolite interactions with collagen and fibrinogen were measured using SPR. Quercetin, apigenin and catechin and tamarixetin, over a range of concentrations (9.375; 18.75; 37.5; 75; 150 × 10−6 M), were perfused for 180 s (30 µL·min−1) over collagen (100 µg·mL−1) or fibrinogen (100 µg·mL−1) immobilized on a dextran matrix. Association of the compounds was monitored over 180 s and dissociation was measured over 300 s in real-time. The baseline represented the response achieved after the subtraction of DMSO alone and flavonoid and metabolite responses following perfusion over the dextran matrix. Affinity (KD) was calculated from association (Ka) and dissociation (Kd) constants derived by fitting response units to the 1:1 Langmuir binding model.
SPR, surface plasmon resonance.