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. 2009 Jul;26(7):979–993. doi: 10.1089/neu.2008.0762

FIG. 3.

FIG. 3.

Spatial and temporal expression of Hsp70, HemeOx1, and GFP following SCI using Q-RT-PCR. Hsp70 expression was measured in the P1, I, and D1 segments at 6, 12, and 24 h post-injury. RG3.6-treated spinal cord showed significant up-regulation of Hsp70 as early as 6 h following SCI and the expression declined at 24 h after injury. HemeOx1 was increased in I and D1 segments of RG3.6-treated spinal cord compared to medium controls at 12 h. The distribution of RG3.6 cells was measured by GFP expression. GFP was detected in I segments, while very limited signal was found in P1 or D1 segments at 6–12 h following SCI. By 24 h post-injury, GFP signals were distributed to P1 and D1 segments. Scale represents fold change after normalizing expression to GAPDH. Values are means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA.