FIG. 5.

Spatial and temporal expression of Foxg1 and Top2a following SCI by Q-RTPCR. Transcription factor Foxg1 showed specific and significant up-regulation in RG3.6-treated spinal cord; the expression lasted up to 24 h post-injury (A). Foxg1 immunostaining at 24 h SCI showed strong expression at the dorsal white matter (∼2.5 mm distal to injury epicenter) and did not overlap with RG3.6 cells (B) shown in green. Medium-treated spinal cords showed very weak Foxg1 expression (arrows in B). DNA replication enzyme Top2a was also up-regulated significantly in RG3.6-treated spinal cords by 6 h post-injury by comparison to medium controls in which the expression was not significantly induced until 24 h post-injury (C). Expression levels represent fold change after normalizing to GAPDH. Values are means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA. Scale bar = 100 μm (B); Luxol fast blue stained cross-section illustrates Foxg1 staining region.