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. 2009 Jul;26(7):979–993. doi: 10.1089/neu.2008.0762

FIG. 7.

FIG. 7.

Spatial and temporal expression of Nkx2.2 following SCI. Nkx2.2 was upregulated after SCI; the level was higher in RG3.6 than in control treated spinal cord at 12 h post-injury (A). Immunostaining of Nkx2.2 antibody at 24 h post-SCI showed significantly higher numbers of Nkx2.2-positive cells in RG3.6-treated spinal cords than with medium treatment (B). Nkx2.2-positive cells were quantitated in dorsal and ventral white matter in para-sagittal sections close to the midline (red lines 4, 5, 6 in the schematic spinal cord; C). Spinal cords with RG3.6 transplants showed significantly higher numbers of Nkx2.2 cells in rostral and caudal white matter than with medium treatment (C). In RG3.6-treated spinal cords, 54% of Nkx2.2 cells were NG2 positive (Cy5 shown in green) (D). Expression levels in A represent fold change after normalizing to GAPDH. Values are means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA. Values in C are means ± SEM, *p < 0.05, **p < 0.01, t-test (C). Scale bar = 20 μm (B,D).