Table 1.
Antibody List
| Primary antibody | Dilution | Isotype | Source | Secondary antibody | Dilution | Source |
|---|---|---|---|---|---|---|
| Iba1 (ionized calcium-binding adaptor molecule 1) | 1: 1000 | Rabbit Polyclonal IgG | Wako | Alexa568 Goat-anti-rabbit IgG (H + L) | 1: 400 | Molecular Probe |
| ED1 (CD68) | 1: 300 | Mouse Monoclonal IgG | Serotec | Alexa568 Goat-anti-mouse IgG (H + L) | 1: 400 | Molecular Probe |
| Foxg1 | 1: 1000 | Rabbit Polyclonal IgG | Dr. Lorenz Studer | Alexa568 Goat-anti-rabbit IgG (H + L) | 1: 400 | Molecular Probe |
| Nkx2.2 | 1: 20 | Mouse Monoclonal IgG | DSHB | Alexa568 Goat-anti-mouse IgG (H + L) | 1: 400 | Molecular Probe |
| NG2 | 1: 500 | Rabbit Polyclonal IgG | Dr. Joel Levine | Alexa647 (Cy5) Goat-anti-rabbit IgG (H + L) | 1: 400 | Molecular Probe |
| Vimentin | 1: 20 | Mouse Monoclonal IgM | DSHB | Alexa568 Goat-anti-mouse IgM | 1: 400 | Molecular Probe |