Figure 5.

Effect of POLQ siRNA on normal cells. A) POC cells treated with POLQ siRNA were not sensitised to IR. B) MRC5 cells show modest sensitisation to POLQ depletion only at high doses of IR. **, P < 0.01 unpaired two sided t-test. C) Effective knockdown of POLQ in MRC5 cells treated with 50nM POLQ siRNA as determined by qRT-PCR. D) Relative expression of POLQ in untransfected T24 and MRC5 cells as determined by qRT-PCR. The expression of POLQ was expressed as a ratio relative to the presence of GAPDH in each cell line.